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当前位置: 首页 > 在线留言 > 1. What is the difference between this DNA modification kit and your "one-step" DNA modification kit (Cat #P-1010)?
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  • question 1. What is the difference between this DNA modification kit and your "one-step" DNA modification kit (Cat #P-1010)?

    • answer In the one-step DNA modification, DNA is denatured by heating, which allows DNA denaturation and bisulfite conversion to be carried out simultaneously. One-step DNA modification is suitable for the labs which are equipped with thermal cycler and requires simple and fast procedures for DNA modification. In the "two-step" DNA modification of this kit, DNA is denatured chemically followed by bisulfite treatment. It is suitable for general DNA modification using DNA isloated from various sources. Because one-step DNA modification may increase DNA degradation, a higher starting DNA amount may be required for one-step DNA modification than for two-step DNA modifcation.
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