Epigentek/MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)/P-1037-96/96 assays,Epigentek,,Epigentek,Epigentek Group Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Epigentek/MethylFlash Hydroxymethylated DNA Quantification Kit (Fluorometric)/P-1037-96/96 assays

产品编号：P-1037-96

市场价：¥12380.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$619.00

品牌： Epigentek

公司：Epigentek Group Inc.

公司分类：

商品介绍

InputType:	DNA
ResearchArea:	DNAMethylation
TargetApplication:	AmountQuantitation
VesselFormat:	96-WellPlate
100%Guarantee:	6months

ProductOverview

TheMethylFlash™HydroxymethylatedDNAQuantificationKit(Fluorometric)isacompletesetofoptimizedbuffersandreagentstofluorometricallyquantifyglobalDNAhydroxymethylationbyspecificallymeasuringlevelsof5-hydroxymethylcytosine(5-hmC)withoutcross-reactivitytomethylcytosineandunmethylatedcytosine.

Thiskitisalsospecificallyoptimizedforpairedusewiththe MethylFlashMethylatedDNAQuantificationKit(Fluorometric) forsimultaneouslyquantifyingbothmethylatedDNAandhydroxymethylatedDNA.

About5-Hydroxymethylcytosine
5-hmCisamodifiedformofcytosine,recentlydiscoveredinanimaltissues.Thefunctionof5-hmCinepigeneticsmaybedifferentfromitsforerunner5-methylcytosine(5-mC)andcurrentlyremainsamystery.Itisbelieved,though,that5-hmCplaysanimportantroleinswitchinggenesonandoff. Thepresenceof5-hmCmakesitnecessarytonotonlyre-evaluateexistingDNAmethylationdata,butalsonecessarytodeterminetherelativedistributionandchangesof5-hmCinhumantissuesofhealthyanddiseasedstatuses.PriortoEpigentek’sMethylFlash™ technology,therewerenomethodsthatcouldbeusedforpracticallyandroutinelyidentifying5-hmCanddiscriminatingthisbasefrom5-mC.

DistinguishingBetween5-hmCand5-mC
CurrentlyusedmethylatedDNAanalysismethodsincludingrestrictionenzymedigestionandbisulfiteorMeDIP-mediatedMS-PCRandsequencingarenotsuitablefor5-hmCdetectionas5-hmCand5-mCarevirtuallyindistinguishablebetweeneachotherwiththesemethods.Toaddressthisproblem,EpigentekofferstheMethylFlash™HydroxymethylatedDNAQuantificationKit(Fluorometric)whichusesauniqueproceduretoquantifyglobalDNAhydroxymethylation. Thisproductprovidesacost-effectivewaytomeasurelevelsof5-hydroxymethylcytosineandtodistinguishbetween5hmC,5mC,andC.Thisallowsforresearcherstore-evaluatetheirDNAmethylationdataforDNAhydroxymethylationandtoefficientlylookforDNAhydroxymethylationinnewDNAsamples. Thekithasthefollowingadvantagesandfeatures:

Fluorometricassaywitheasy-to-followstepsforconvenienceandspeed.Theentireprocedurecanbecompletedwithin3hoursand20minutes.
Highsensitivity,ofwhichthedetectionlimitcanbeaslowas10pgofhydroxymethylatedDNAand acceptsaslowas20ngofinputgenomicDNA
Highspecificitywithnocross-reactivityto methylcytosineand unmethylatedcytosine.5-hydroxymethylcytosineisseparatelydetected.
Universalpositiveandnegativecontrolsareincluded,whicharesuitableforquantifyinghydroxymethylatedDNAfromanyspecies.
Strip-wellmicroplateformatmakestheassayflexIBLeforeithermanualorhighthroughputanalysis.
Simple,reliable,andconsistentassayconditions.

ComparisonofAvailableELISA-basedGlobalDNAMethylationAssayMethods

	MethylFlash (Fluorometric)	CompetitorZ
AssayPrinciple	DirectELISA-Detectionantibodyspecificallyanddirectlydetects5-hmC	IndirectELISA-DetectionantibodydetectsdsDNA,(detectedsignalsisdependentonlyonintactDNAstrandsanddoesnotquantifyactualtotal5-hmC)
Format	96-wellplate	96-wellplate
Sensitivity	Excellentdetectionlimitof0.01ngof5-hmCDNA	Unclear,asthedetectedsignalsarefromintactDNAstrands
S/NRatio	>20withverylowbackground	<4withhighbackground
ProceduralConvenience	NoneedforplateblockinganddenaturationofDNA	RequiresplateblockingandDNAdenaturation
ProtocolTime	<4hr	<5hr
DNAType&Species	Universalforanyspecies,bothssDNAanddsDNA	OnlyssDNAfromlimitednumberofspecies
Specificity	High,theantibodyonlydetects5-mC	Unclear,astheassayprincipleisnotreasonable
QuantificationType	Bothrelativeandabsolutequantification	Relativeonly
StandardControl	Stable,quantitativeinabsoluteamountof5-mC,andcanbeuniversallyusedforanyspecies	Unclear
MinimumInputAmount	20ng	100ng
AccuracyofDetection	StableandquantifiedstandardcontrolwithprovenclosecorrelationwithHPLC-MSanalysisbyusers	Unclear,basedonbiasedassayprinciple
%5-mCCalculation	Simple	Complicated
PatentedMethod	Yes	No
Popularity	Veryhighpublishedcitationcount	Lowpublishedcitationcount
SupportExpertise	Since2006	Since2012

Fig.1.SchematicprocedureoftheMethylFlash™HydroxymethylatedDNAQuantificationKit.

ProductComponents

HF1(10XWashBuffer)
HF2(BindingSolution)
HF3(NegativeControlI,20µg/ml)*
HF4(NegativeControlII,20µg/ml)*
HF5(PositiveControl,20µg/ml)*
HF6(CaptureAntibody,1000µg/ml*
HF7(DetectionAntibody,400µg/ml)*
HF8(EnhancerSolution)*
HF9(FluoroDeveloper)*
HF10(FluoroEnhancer)*
HF11(FluoroDilutor)
8-WellAssayStrips(WithFrame)
UserGuide

*Spinthesolutiondowntothebottompriortouse.

Note:TheHF3NegativeControlIisanunmethylatedpolynucleotidecontaining20%ofcytosine.TheHF4NegativeControlIIisamethylatedpolynucleotidecontaining20%of5-methylcytosine.TheHF5PositiveControlisahydroxymethylatedpolynucleotidecontaining20%ofhydroxymethylcytosine.

品牌介绍

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公司简介
作为表观遗传学公司，Epigentek Group Inc.是表观遗传相关研究的创新技术和产品的领先开发商和提供商。自2005年成立以来，该公司已经开发了一系列专有产品，可为表观遗传学相关的研究和药物发现提供完整，系统的解决方案。

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