Epigentek/MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)/P-1039-96/96 assays,Epigentek,,Epigentek,Epigentek Group Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Epigentek/MethylFlash Urine 5-Methylcytosine (5-mC) Quantification Kit (Colorimetric)/P-1039-96/96 assays

产品编号：P-1039-96

市场价：¥10180.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$509.00

品牌： Epigentek

公司：Epigentek Group Inc.

公司分类：

商品介绍

InputType:	Urine
ResearchArea:	DNAMethylation
TargetApplication:	AmountQuantitation
VesselFormat:	96-WellPlate
100%Guarantee:	6months

ProductOverview

TheMethylFlash™Urine5-Methylcytosine(5-mC)QuantificationKit (Colorimetric) isacompletesetofoptimizedbuffersandreagentstocolorimetricallyquantify5-mCinurineusinganinhibitorycompetitiveimmunoassaymethod.It issuitablefordetectingtotalurinary5-methylcytosinelevels,resultingfromwholebodyturnoverordegradationofmethylatedDNA/RNA,inurinefromhumansandanimals.Theurinesamplescanbeinfreshorfrozenform. Thekithasthefollowingadvantagesandfeatures:

Innovativecolorimetricassaywitheasy-to-followstepsforconvenienceandspeed.Theentireprocedurecanbefinishedwithin4hours.
96strip-wellmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis.
Innovativekitcompositionenablesbackgroundsignalstobeextremelylow,whicheliminatestheneedforplateblockingandallowstheassaytobesimple,accurate,reliable,andconsistent.
Thelevelof5-mCmeasuredinhumanurinesamplesusingthiskitiscomparabletothatdetectedbyHPLCmethod.
Anovelassayprincipleallowshighsensitivitytobeachieved.Thedetectionlimitcanbeaslowas0.03ng/assaywellor1nMof5-mC.
Lowinputrangeofurineforeachassaywithavolumeof0.5to5µlandanoptimalvolumeof1µl.
Optimizedantibodyandenhancersolutionsallowforhighspecificityto5-mC,withoutcross-reactivitytounmethylatedcytosine.
Negativecontrolandpositivestandardareincluded,whicharesuitableforquantificationof5-mCinfreeformand5-mCcontainedinmethylatedDNA/RNAfragmentsfromdifferenturinesamples.

BackgroundInformation
Nucleobase5-methylcytosine(5-mC),amodifiedformofcytosineconvertedbycytosinemethyltransferases,iswidespreadbothinDNAanddifferentcellularRNAs.TheBIOLOGicalimportanceofDNA5-mCmethylationasamajorepigeneticmodificationinphenotypeandgeneexpressionhasbeenwidelyrecognized.RecentdatastronglysuggeststhatRNA5-mCmethylationisalsoinvolvedintheregulationofvariousbiologicalprocessesincludingtRNAstABIlity/recognitionandmRNAtranslation.

Urinaryexcretionof5-mCincludingboth5-methyl-2-deoxycytidineand5-methylcytidineisanindicationofawholebodyturnoverordegradationofmethylatedDNAandRNA.Theurinary5-mClevelcanbealteredbyachangeofthebodies’turnoverofmethylatedDNA/RNAoralterationofcellularDNA/RNAmethylationstatus.Anumberofstudieshaveindicatedthat5-mCexcretedinurinehasthepotentialtoactasacancerbioMarker,withanincreasedlevelarisingfromdiseaseonsetandprogression.Forexample,anelevatedlevelofurinary5-mCwasobservedinlungcancer,breastcancer,andleukemiapatientswithactivediseasestates.Itwasalsoshownthaturinary5-mCexcretioniseffectedbyAlzheimer’sdiseaseorbyrADIationtreatment.Itissuggestedthaturinary5-mCmightbeapplicableasabiologicalmarkerfordetectingsometypesofcancerandmonitoringcancerprogressionaftertreatmentwithradiationordemethylationreagents.

Chromatography-basedtechniquessuchasHPLCandTLCmassspectrometryarecommonlyusedfordetecting5-mCinurineandalthoughfairlyacurate,aretimeconsuming,lesssensitive,andhavelowthroughputwithhighcosts.Toaddressthisproblem,EpigentekofferstheMethylFlash™ Urine5-Methylcytosine(5-mC)QuantificationKit (Colorimetric) toquantify5-mCorbodyturnoverstatusofmethylatedDNA/RNAusingurinesamples.

Principle&Procedure
InthisELISA-likeinhibitorycompetitiveimmunoassayassay,urinesamplesandthe5-mCstandardarefirstincubatedwitha5-mCantibodysolutionandthentransferredtostripwellscoatedwithmethylatedDNA(5-mC).Thewelliswashedtoremoveanyunboundreagentsafterincubationandthenadetectionantibodyisaddedtogenerateasignalthatcanbemeasuredcolorimetricallybyreadingtheabsorbanceinamicroplatespectrophotometer.Because5-mCintheurinesampleinhibitsthebindingof5-mCantibodyto5-mCcoatedonthewell,higherconcentrationsof5-mCintheurinesampleleadtoareducedbindingoftheantibodytothe5-mConthewell.ThereforethesignalorODintensitymeasuredfromthewellwillbeinverselyproportionaltotheamountof5-mCintheurinesampleandtheamountof5-mCintheurinesamplecanbequantifiedbyacomparisonwithapredetermined5-mCstandard.

Fig.1.SchematicprocedurefortheMethylFlash™Urine5-MethylcytosineQuantificationKit(Colorimetric).

Fig.2. 5-mCstandardwasaddedintotheassaywellsatdifferentconcentrationsandthenmeasuredwiththeMethylFlash™Urine5-MethylcytosineQuantificationKit (Colorimetric).

Fig.3. 5-mClevelisquantifiedfromdifferentvolumesofhumanurineusingtheMethylFlash™Urine5-MethylcytosineQuantificationKit (Colorimetric).

ProductComponents

WB(10XWashBuffer)
BS(BindingSolution)
MS(5-mCStandard,4µg/ml)*
ME3(NegativeControl,50µg/ml)*
ME4(5-mCDNAsolution,320X)*
ME5(CaptureAntibody,1000X)*
ME6(DetectionAntibody,2000X)*
ES(EnhancerSolution)*
DS(DeveloperSolution)
SS(StopSolution)
Plate1(AssayPlate)(WithFrame)
Plate2(SamplePreparationPlate)
UserGuide

*Spinthesolutiondowntothebottompriortouse.

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