Epigentek/Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric)/P-3087-96/96 assays,Epigentek,,Epigentek,Epigentek Group Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Epigentek/Epigenase 5mC-Hydroxylase TET Activity/Inhibition Assay Kit (Fluorometric)/P-3087-96/96 assays

产品编号：P-3087-96

市场价：¥11380.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$569.00

品牌： Epigentek

公司：Epigentek Group Inc.

公司分类：

商品介绍

InputType:	NuclearExtracts,PurifiedEnzyme
ResearchArea:	DNAMethylation
TargetApplication:	ActivityMeasurement
VesselFormat:	96-WellPlate
100%Guarantee:	6months

ProductOverview

TheEpigenase™ 5mC-HydroxylaseTETActivity/InhibitionAssayKit(Fluorometric)isacompletesetofoptimizedbuffersandreagentsformeasuringactivity/inhibitionoftotalcytosineoxygenaseTETenzymesinnuclearextractsorpurifiedTETisoforms(TETs1-3)fromabroadrangeofspeciessuchasmammals,plants,fungi,andbacteria,andinavarietyofformsincluding,butnotlimitedtoculturedcellsandfreshandfrozentissues. Thekithasthefollowingadvantagesandfeatures:

5hourfluorometricassayprocedurewitheasy-to-followstepsforconvenienceandspeed.Theentireprocedurecanbefinishedwithin5hours.
DirectlymeasuresTEThydroxylaseactivityviaastraightforwarddetectionofTET-convertedhydroxymethylatedproducts.
Innovativekitcompositionenablesbackgroundsignalstobeextremelylowandallowstheassaytobesimple,accurate,reliable,andconsistent.
Eithercell/tissueextractsorpurifiedTETproteinscanbeused,whichallowsfordetectionofinhibitoryeffectsofTEThydroxylaseinhibitorsinvivo and invitro.
Novelassayprincipleallowshighsensitivitytobeachieved.Theactivitycanbedetectedfromaslowas10ngofpurifiedTET1hydroxylase.
Ahydroxymethylatedstandardisincluded,whichallowsthespecificactivityofTEThydroxylasestobequantified.
StripmicroplateformatmakestheassayflexIBLeformanualorhighthroughputanalysis(96assays).

BackgroundInformation
5-hydroxymethylcytosine(5hmC),asasixthDNAbasewithfunctionsintranscriptionregulation,hasbeendetectedtobeabundantinthehumanandmousebrainandembryonicstem(ES)cells.Inmammals,itcanbegeneratedbyoxidationof5-methylcytosine,areactionmediatedbytheten-eleventranslocation(TET)familyofcytosineoxygenases.

TheTETfamilyofcytosineoxygenasesincludesTET1,TET2,andTET3.TheseTETproteinsmaypromoteDNAdemethylationbybindingtoCpG-richregionstopreventunwantedDNAmethyltransferaseactivity,andbyconverting5mCto5hmC andfurtherto5caC(5-carboxylcytosine) throughhydroxylaseactivity.Itwasshownthatgenomic5hmClevelscorrelatewithTEThydroxylaseactivity.Inaddition, TET1wasshowntohavedualfunctionsintranscriptionactivationandrepressionbybindingdifferenttargetgenesinEScells.TET1isalsoafusionpartneroftheMLLgeneinacutemyeloidleukemiaandisconsideredanoncoprotein.TET2isfoundtobefrequentlymutatedinleukemiaandconsideredtoactastumorsuppressor.TET3wasdemonstratedtoplayauniqueroleinDNAmethylationreprogrammingprocessesinthemammalianzygote.Thus,activatingtumorsuppressorTETenzymessuchasTET2orinhibitingoncoproteinTETenzymessuchasTET1wouldbeimportantinbenefitingcancerdiagnositcsanddevelopingnewtarget-basedcancertherapeutics.

Principle&Procedure
Inthisassay,amethylatedsubstrateisstablycoatedontothemicroplatewells.ActiveTETs(notincluded)bindtothesubstrateandconvertmethylatedsubstratehydroxymethylatedproductsfromthesubstrate.TheTET-convertedhydroxymethylatedproductscanberecognizedwithaspecificantibody.Theratiooramountofhydroxymethylatedproducts,whichisproportionaltoenzymeactivity,canthenbefluorometricallymeasuredbyreADIngthefluorescenceinafluorescentmicroplatereaderat530excitationand590emission.TheactivityoftheTETenzymeisinturnproportionaltotherelativefluorescentunitsmeasured.

Fig.1. SchematicprocedurefortheEpigenase™5mC-HydroxylaseTETActivity/InhibitionAssayKit(Fluorometric).

Fig.2. DemonstrationofhighsensitivityandspecificityoftheTET1activity/inhibitionassayachievedbyusingrecombinantTET1withtheEpigenase5-mCHydroxylaseTETActivity/InhibitionAssayKit(Fluorometric).

Fig.3. DemonstrationofhighsensitivityandspecificityoftheTETactivityassayachievedbyusingnuclearextractswiththetheEpigenase5-mCHydroxylaseTETActivity/InhibitionAssayKit(Fluorometric).NuclearextractswerepreparedfrommouseEScellsbyusingtheEpiQuikNuclearExtractionKit(Cat.No.OP-0002).

Fig.4. Illustratedstandardcurvegeneratedwiththekit"sTETassaystandard.

ProductComponents

WBWashBuffer
TABTETAssayBuffer
TS10XTETSubstrate
BSBindingSolution
HC5TETAssayStandard
HC6CaptureAntibody
HC7DetectionAntibody
HC8EnhancerSolution
FDFluoroDeveloper
FEFluoro-Enhancer
Co-factor1
Co-factor2
Co-factor3
8-WellAssayStrips(WithFrame)

品牌介绍

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公司简介
作为表观遗传学公司，Epigentek Group Inc.是表观遗传相关研究的创新技术和产品的领先开发商和提供商。自2005年成立以来，该公司已经开发了一系列专有产品，可为表观遗传学相关的研究和药物发现提供完整，系统的解决方案。

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该公司特别强调其技术的速度和有效性，继续关注表观遗传创新，并广泛开发和增强其产品组合。