请使用支持JavaScript的浏览器!
主营:基因表达
℡ 4000-520-616
℡ 4000-520-616
Epigentek/Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)/P-3094-96/96 Assays
产品编号:P-3094-96
市  场 价:¥11780.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$589.00
品      牌: Epigentek
公      司:Epigentek Group Inc.
公司分类:
Epigentek/Epigenase Thymine DNA Glycosylase (TDG) Activity/Inhibition Assay Kit (Colorimetric)/P-3094-96/96 Assays
商品介绍
InputType:NuclearExtracts,PurifiedEnzyme
ResearchArea:DNAMethylation
TargetApplication:ActivityMeasurement
VesselFormat:96-WellPlate
100%Guarantee:6months
ProductOverview

TheEpigenase™ThymineDNAGlycosylase(TDG)Activity/InhibitionAssayKit(Colorimetric)isacompletesetofoptimizedbuffersandreagentsformeasuringtheactivity/inhibitionoftotalTDGenzymeinnuclearextractsorpurifiedTDGenzymesfromabroadrangeofspeciessuchasmammals,plants,fungi,andbacteria,inavarietyofformsincludingbutnotlimitedtoculturedcellsandfreshandfrozentissues.Thekithasthefollowingadvantagesandfeatures:

  • Colorimetricassaywitheasy-to-followstepsforconvenienceandspeed.Theentireprocedurecanbefinishedwithin5hours.
  • DirectlymeasuresTDGactivitywithouttheneedforDNAcleavage,electrophoresis,orchromatography. 
  • Bothcell/tissueextractsandpurifiedTDGproteinscanbeused,whichallowsfordetectionofinhibitoryeffectsofTDGinhibitorsinvivoandinvitro.
  • Novelassayprincipleallowshighsensitivitytobeachieved.Theactivitycanbedetectedfromaslowas20ngofpurifiedTDGprotein.
  • TDGassaystandardisconvenientlyincluded,whichallowsforspecificquantificationofTDGactivity. 
  • StripmicroplateformatmakestheassayflexIBLe:manualorhighthroughputanalysis(96assays).
BackgroundInformation
DNAdemethylationhasbeendemonstratedtooccurthroughatleasttwodifferentpathways:(1)activedeaminationof5-mCtoTbyanAID/APOBECenzyme,givingaG/TmismatchthatisconvertedtoG/CbythymineDNAglycosylase(TDG)andthensubsequentlybaseexcisionrepair(BER);and(2)iterativeoxidationof5-mCbyTETenzymeto5-hmCandfurtherto5-formycytosine(5-fC)and5-carboxylcytosine(5-caC).Both5-fCand5-caCcanberapidlyexcisedbyTDGtoallowsubsequentBERprocessingwhichresultsin5-fCand5-caCconvertingbacktounmodifiedcytosine.

TDGbelongstotheTDG/mugDNAglycosylasefamily.BesidesplayingacriticalroleinactiveDNAmethylation,TDGalsoremovesthyminemoietiesfromG/Tmismatchesbyhydrolyzingthecarbon-nitrogenbondbetweenthesugar-phosphatebackboneofDNAandthemismatchedthymine.IthasbeenshownthatTDGparticipatesintheregulationofembryonicandgermcelldevelopment,mediatescellulardefenseagainstgeneticandepigeneticmutations,andmediatesDNA-directedcytotoxicity.Inaddition,TDGisaproposedtumorsuppressorcandidate.Thus,themeasurementofTDGactivitywouldbeimportantasTDGmaybeinvolvedinpreventingtumOrigenesisanddeterminingcellularresponsetochemotherapythroughDNAdemethylationandBER,therebybenefitingCancerdiagnosticsandnewtarget-basedcancertherapeutics. 

ThecurrentlyusedmethodsforTDGactivitydetectioninvolveDNAcleavagefollowedbyelectrophoresis,whichcanbetimeconsumingandinconvenient,withlowthroughputandhighcost.Toaddressthisissue,EpigentekdevelopedandofferstheEpigenase™ThymineDNAGlycosylase(TDG)Activity/InhibitionAssayKit(Colorimetric).

Principle&Procedure
Inthisassay,a5-fCDNAsubstrateisstablycoatedontomicroplatewells.ActiveTDGbindstothesubstrateandexcises5-fCfromthesubstratebyanickingreaction.Theremainingun-excised5-fCinthesubstratewillberecognizedwithahighaffinity5-fCDNAantibody.Theratiooramountof5-fCinthesubstrate,whichisinverselyproportionaltoenzymeactivity,canthenbecolorimetricallymeasuredbyreADIngtheabsorbanceinamicroplatespectrophotometeratawavelengthof450nm.TheactivityoftheTDGenzymeisinturninverselyproportionaltotheopticaldensityintensitymeasured.

StartingMaterials&InputAmount
Theamountofnuclearextractsforeachassaycanbe2µgto20µgwithanoptimalrangeof5-6µg.Theamountofpurifiedenzymescanbe20ngto1µg,dependingonthepurityandcatalyticactivityoftheenzymes.


Fig.1.SchematicprocedureoftheEpigenase™ThymineDNAGlycosylase(TDG)Activity/InhibitionAssay(Colorimetric). 


Fig.2. IllustratedstandardcurvegeneratedwiththeTDGassaystandardfromthe Epigenase™ThymineDNAGlycosylase(TDG)Activity/InhibitionAssay(Colorimetric).


Fig.3. DemonstrationofhighsensitivityandspecificityoftheTDGactivityassayachievedbyusingnuclearextractswiththetheEpigenase™ThymineDNAGlycosylase(TDG) Activity/InhibitionAssayKit(Colorimetric).HelaNuclearextractswereusedintheassay.

 

 

ProductComponents

WB(10XWashBuffer)
TAB(TDGAssayBuffer)
BS(BindingSolution)
TS(10XTDGSubstrate)*
TAS(TDGAssayStandard,10µg/ml)*
CA(CaptureAntibody,1000X)*
DA(DetectionAntibody,2000X)*
ES(EnhancerSolution)*
DS(DeveloperSolution)
SS(StopSolution)
8-WellAssayStrips(WithFrame)
UserGuide

*Spinthesolutiondowntothebottompriortouse.

品牌介绍

Epigentek我们的使命
通过创新和智能的研究,建立并成为表观遗传相关产品,技术和服务的全球领先,完整和系统的提供商。

我们的目标是:

  • 协助研究人员解决表观遗传问题中的挑战。

  • 开发表观遗传实验并将其简化并引入到表观遗传实验中。

  • 通过出色的可靠性,速度,服务和质量来确保客户满意度。

  • 鼓励并促进表观遗传学的研究,以用于潜在的医学应用。

  • 通过对技术趋势的常规分析来开拓和创新表观遗传产品。

公司简介
作为表观遗传学公司,Epigentek Group Inc.是表观遗传相关研究的创新技术和产品的领先开发商和提供商。自2005年成立以来,该公司已经开发了一系列专有产品,可为表观遗传学相关的研究和药物发现提供完整,系统的解决方案。

Epigentek产品在全球享有盛誉的学术界,领先的制药公司和尖端生物技术公司广泛使用。这些独特的产品经过专门设计,可以使检测比当前使用的方法更简单,更快,更方便且更高效。Epigentek的产品通过遍布36个国家和地区的专业销售团队和全球分销商网络进行销售。确保我们技术的创新将使客户完全满意并在您的实验中获得最大的成功是我们的第一要务。

该公司特别强调其技术的速度和有效性,继续关注表观遗传创新,并广泛开发和增强其产品组合。


自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔