Epigentek/EpiQuik In Vivo Protein Sumoylation Assay Ultra Kit (Colorimetric)/P-8003-48/48 assays,Epigentek,,Epigentek,Epigentek Group Inc,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Epigentek/EpiQuik In Vivo Protein Sumoylation Assay Ultra Kit (Colorimetric)/P-8003-48/48 assays

产品编号：P-8003-48

市场价：¥6780.00

场地：美国(厂家直采)

产品分类：生化试剂>缓冲液>酸碱缓冲液>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$339.00

品牌： Epigentek

公司：Epigentek Group Inc.

公司分类：

商品介绍

InputType:	NuclearExtracts
ResearchArea:	Sumoylation
TargetApplication:	AmountQuantitation
VesselFormat:	96-WellPlate
100%Guarantee:	6months

ProductOverview

The EpiQuik™InVivoProteinSumoylationAssayUltraKit isacompletesetofoptimizedreagentsdesignedformeasuringinvivoproteinSUMOylationfrommultiplemammaliancells/tissuesincludinghuman,mouse,andrat. Thekithasthefollowingadvantages:

Fastprocedure,whichcanbefinishedwithin5hours.
DirectcolorimetricassaywithouttheneedforaffinitychromatographyorWesternblot.
FlexIBLechoiceofantibodyofinterestallowsforthedetectionofSUMOylationofmultipletargetproteinssimultaneously.
Useofoptimizeddetectionantibodyeliminatesthestepfordetectionsolutionpreparation,therebyincreasingdetectionsensitivityandassayconvenience.
Thepositivecontrol(SUMOprotein)andnegativecontrol(un-SUMOylatednon-immuneIgGprotein)allowsproteinSUMOylationtobequantified.
Stripmicroplateformatmakestheassayflexible:manualorhighthroughputanalysis.
Reliableandconsistentassayconditions.

BackgroundInformation
SUMOylationisapost-translationalmodificationinvolvedinvariouscellularprocesses,suchasnuclear-cytosolictransport,transcriptionalregulation,apoptosis,proteinstABIlity,responsetostress,andprogressionthroughthecellcycle.SUMOproteinsaresimilartoubiquitin.Thereare3confirmedSUMOisoformsinhumans:SUMO-1,SUMO-2,andSUMO-3.SUMO-2/3showahighdegreeofsimilaritytoeachotherandaredistinctfromSUMO-1.SUMOylationisdirectedbyanenzymaticcascadeanalogoustothatinvolvedinubiquitination.SUMOylationoftargetproteinsinvivohasbeenshowntocauseanumberofdifferentoutcomes,includingalteredlocalizationandbindingpartners.DetectionofinvivoproteinSUMOylation(SUMOconjugation)wouldprovideusefulinformationforunderstandingSUMOmodificationthatemergesasanimportantcontrolmechanismregulatingtheactivityofmanynuclearproteins.

Principle&Procedure
Inanassaywiththiskit,theantibodiesspecifictothetargetedproteinsarestablyboundtothestripwellsandthetargetedproteinsarecapturedbytheseantibodies.SUMOylationofthetargetedproteinsaredetectedbyrecognitionofSUMOconjugatedtotheseproteinswithananti-SUMOantibody.TheratioorintensityoftheSUMOylation,whichisproportionaltotheconjugatedSUMOamount,canbequantifiedthroughthesignalreport-colordevelopmentsystembyusingamicroplatereaderat450nmwavelength.

StartingMaterials
Inputmaterialisnuclearextract.Theamountofnuclearextractsforeachassaycanbe1µgto20µgwithanoptimalrangeof5to10µg.

Fig.1.SchematicProcedureforUsingtheEpiQuik™InVivoProteinSumoylationAssayUltraKit(Colorimetric).

Fig.2. IllustratedstandardcurvegeneratedwithSUMOproteincontrol.

ProductComponents

WB(10XWashBuffer)
BS(BindingSolution)
NC(NegativeControl,250µg/ml)*
BB(BlockingBuffer)
SAB(SUMOAssayBuffer)
PC(PositiveControl,1µg/µl)*
DA(DetectionAntibody,1000X)*
CD(ColorDeveloper)
SS(StopSolution)
8-WellAssayStrips(WithFrame)
UserGuide

*Spinthesolutiondowntothebottompriortouse.

品牌介绍

Epigentek我们的使命
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公司简介
作为表观遗传学公司，Epigentek Group Inc.是表观遗传相关研究的创新技术和产品的领先开发商和提供商。自2005年成立以来，该公司已经开发了一系列专有产品，可为表观遗传学相关的研究和药物发现提供完整，系统的解决方案。

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该公司特别强调其技术的速度和有效性，继续关注表观遗传创新，并广泛开发和增强其产品组合。