The EpiQuik™ HDAC8 Assay Kit is a convenient package of tools that allows the experimenter to measure the amount of HDAC8 at tremendously fast speeds and consistency; superior and safer than all other current methods. The kit is ready-to-use and provides all the essential components needed to carry out a successful HDAC8 assay experiment without the need for electrophoresis and transfer process. It is suitable for measuring HDAC8 level from various fresh tissues and cultured mammalian cells. The kit has the following advantages:

• Fast procedure, which can be finished within 3 hours.
• Innovative colorimetric assay to semi-quantitatively measure HDAC8 amount without the need for electrophoresis.
• Strip microplate format makes the assay flexible: manual or high throughput analysis.
• Simple, reliable, and consistent assay conditions.

Principle & ProcedureThe EpiQuik™ HDAC8 Assay Kit is designed for measuring total HDAC8 amount from tissues or cells. In an assay with this kit, the nuclear proteins containing HDAC8 are stably coated on the strip wells. The HDAC8 is recognized with a high-affinity specific antibody. The amount of HDAC8 can be quantified through a HRP conjugated secondary antibody-color development system and is proportional to the intensity of color development.

Fig. 1. Schematic procedure for using the EpiQuik HDAC8 Assay Kit (Colorimetric).

Fig. 2.HDAC8 proteins were bound to microplate wells at different concentrations and measured using the EpiQuik™ HDAC8 Assay Kit.

Fig. 3.Nuclear proteins were prepared from different human tissue samples. 5 µg of nuclear proteins were bound to microplate wells for HDAC8 level measurement using the EpiQuik™ HDAC8 Assay Kit.